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KMID : 0387719960070020173
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Korean Journal of Blood Transfusion
1996 Volume.7 No. 2 p.173 ~ p.180
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The Rh(D) RBC Antigen Density Measurement by Flowcytometry
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임채승
김영기/이갑노/김대철/황유성/김두성
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Abstract
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Backgrounds:
The Rh antigens are important in clinical practice. The classification of Rh phenotype is usually based on the antigen detection done by conventional serologic method, but it has many limitation such as delicate grading of antigen expression.
Recently, Flowcytometry has been introduced in immunohematology to detect and quantitate cell bound immunoglobulins to assess blood cell antigens and related antibodies. So, we tried these method to detect Rh(D) antigen and measure its density
and
evaluated the possibility of clinical usage.
Materials and Methods:
We performed a flowcytometric analysis for the expression of D antigen in D-positive and weak D group in indirect immune fluorescence assay by using polyclonal antibodies. We measured the intensity of immunofluorescence as a degree of antigen
density and analysed the difference of mean channel fluorescence value (MCF) among these groups.
Results:
Weak D groups had the lower fluorescence than D-positive group, while D-negative sample showed the same fluorecence as negative control. The difference of fluorescence intensity of D antigen between that of C antigen were not observed and
were
statistically insignificant.
Conclusion:
Flowcytometry appear to be a good tool for antigen density measurement in immunohematologic areas and shows the possibility of application to other aspect.
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KEYWORD
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